Platinum-pyrimidine complexes for electron microscopic cytochemistry of deoxyribonucleic acid.

synthesis of binuclear platinum complexes containing biphosphine ligand

هدف از این مطالعه طراحی یک روش کلی برای سنتز یک سری از کمپلکس های دو هسته ای متقارن سیکلومتاله شده پلاتین (ii) بوده که دارای یک لیگاند پل زن(1,2-bis[bis(pentafluoro-phenyl)phosphino]ethane),dfppe, است. از واکنش cis-[pt(r)2(sme2)2]، که در آنr= para-tolyl ، با یک اکی والان از لیگاند benzo[h]quinoline,bhq، در استون محصول [pt(r)(bhq)(sme2)] که در آن r= para-tolyl بدست می آید. همچنین از واکنش 5/0 اک...

Enzymatic Synthesis of Deoxyribonucleic Acid. Iii. the Incorporation of Pyrimidine and Purine Analogues into Deoxyribonucleic Acid.

Cell-surface-associated nucleic acid in tumorigenic cells made visible with platinum-pyrimidine complexes by electron microscopy.

Platinum-pyrimidine complexes have been used as the sole electron-dense stains for electron microscopy on 18 types of mammalian cells. These recently discovered complexes react avidly with nucleic acids in vitro, are antitumor agents, and are highly soluble in water. In cells, they are selective for regions rich in nucleic acid and make visible the cellular chromatin, nucleolus, and ribosomes. ...

Electron microscopic observations on the development and cytochemistry of the large granule complexes in chicken erythrocyte nuclei.

Large granule complexes are structures found in a small percentage of chicken erythrocyte nuclei when observed in ultra-thin sections in the electron microscope. They consist of an amorphous region associated with a number of large (approximately 30 min) granules. We have shown, by a novel use of phenylhydrazine to synchronize populations of chicken erythrocytes in vivo, that large granule comp...

Pyrimidine-specific Antibodies Which React with Deoxyribonucleic Acid (dna).

Progress in a program to synthesize antigens which might be used to elicit antibodies specific for nucleic acid components has been reported.1 In the foregoing paper, the properties and biochemical applications of an antiserum complementary to purin-6-oyl residues were outlined. It was demonstrated, using the complementfixation reaction, that this "anti-purinoyl" antibody cross-reacted with den...